Plin2 Knockout cell line (Raw 264.7)

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Plin2 Knockout cell line (Raw 264.7) Order

Plin2 Knockout cell line (Raw 264.7)
Catalog#
YKO-MT0076
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Plin2 Knockout cell line (Raw 264.7)
Order

Detailed Product Information

Product category
KO Cell Lines
Cell name
Plin2 Knockout cell line (Raw 264.7)
Species
Mouse
Gene Name
Plin2
Gene ID
11520
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 5 (based on transcript Plin2-201)
Validation: PCR+Sanger Sequencing

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Plin2 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Raw 264.7 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Plin2 gene knockout Plin2 Knockout cell line (Raw 264.7) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
Plin2 Knockout cell line (Raw 264.7)
Plin2 Knockout cell line (Raw 264.7)
Catalog#
YKO-MT0076
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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